Total Nucleotide Sequence of Plasmid pKYM and Its Replication Origin
نویسندگان
چکیده
منابع مشابه
The nucleotide sequence of the replication origin of plasmid NTP1.
The sequence of the DNA of the origin region of NTP1 has been obtained. Analysis of the sequence indicates that: (1) there is great sequence homology in the DNA upstream from the origin in NTP1, ColE1, CLODF13, PBR345 AND PBR322; (2) only seven base pairs of NTP1 are identical with the sequence downstream from the origin in ColE1, although some homology exists for 140 bases downstream; (3) two ...
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The replication of the pT181 plasmid is dependent on the plasmid-encoded initiator protein RepC. We have previously shown that RepC protein has sequence-specific endonuclease and topoisomerase-like activities. In this paper we demonstrate that this initiator protein has sequence-specific DNA-binding properties. Based on filter binding of plasmid restriction fragments, RepC protein specifically ...
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It is believed that rolling-circle plasmids are incapable of re-initiation since they have to maintain their copy number and this is one of the differences between plasmids and phages as phi-x174. To examine whether a rolling-circle plasmid pKYM is incapable of re-initiating DNA replication, we constructed a plasmid that carries both the pKYM origin (fragment 13, 173 bp) and its truncated origi...
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The cyanobacteria are an ancient and diverse group of prokaryotes capable of oxygenic photosynthesis and plasmids have been found in both unicellular and filamentous types. Currently, all plasmids are cryptic and only one plasmid, pUH24, from a unicellular non-nitrogen-fixing cyanobacterium, has been sequenced (1). Comparative searches (TFASTA, FASTA (2)) of pUH24 sequences did not show similar...
متن کاملNucleotide sequence of the O gene and of the origin of replication in bacteriophage lambda DNA.
The nucleotide sequence of the O gene in bacteriophage lambda DNA is presented. According to two possible initiator codons, the primary structure of the O protein deduced from the DNA sequence consists of 278 or 299 amino acid residues. Structure and function of the O protein--one of the two phage initiator proteins for lambda DNA replication--are discussed in the light of a secondary structure...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1988
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.108.9_886